ARG10006

anti-IL8 antibody [I8-60]

anti-IL8 antibody [I8-60] for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody

Overview

Product Description

Mouse Monoclonal antibody [I8-60] recognizes IL8

Tested Reactivity Hu
Tested Application ELISA, IHC-P, Neut, WB
Specificity Does not cross react with human Monocyte Chemotactic Activating Factor (MCAF) or RANTES (Regulated on Activation, Normal T-cell Expressed, and Secreted).
Host Mouse
Clonality Monoclonal
Clone I8-60
Isotype IgG1,k
Target Name IL8
Antigen Species Human
Immunogen Purified recombinant human IL-8
Conjugation Un-conjugated
Alternate Names IL8/NAP1 form IV; GCP/IL-8 protein IV; NAF; T-cell chemotactic factor; 1-77; Ala-IL-8; Interleukin-8; IL-8; Neutrophil-activating protein 1; GCP/IL-8 protein II; IL8/NAP1 form II; GCP/IL-8 protein V; MDNCF; Protein 3-10C; Lymphocyte-derived neutrophil-activating factor; Neutrophil-activating factor; Granulocyte chemotactic protein 1; LYNAP; NAP-1; Monocyte-derived neutrophil chemotactic factor; 6-77; 7-77; C-X-C motif chemokine 8; GCP1; NAP1; Ser-IL-8; 5-77; GCP/IL-8 protein VI; IL8/NAP1 form I; IL8/NAP1 form VI; Monocyte-derived neutrophil-activating peptide; C-X-C motif; 8-77; 9-77; LUCT; Chemokine; GCP-1; MDNCF-b; MDNCF-c; IL8/NAP1 form V; LECT; IL8/NAP1 form III; GCP/IL-8 protein III; Emoctakin; GCP/IL-8 protein I; MONAP; IL8

Application Instructions

Application Note ELISA: This antibody can be used as a tracer antibody in sandwich ELISA applications for human IL-8 detection in combination with a monoclonal capture antibody (Cat No: ARG10005).

Neutralizing: In chemotaxis assay, the antibody inhibited 98.4% of the chemotactic activity of IL-8 on RB/293 cell using 5μg/mL of antibody and 10 ng/mL of human IL-8 in assay. This antibody inhibited the chemotactic effect of human IL-8, had no inhibitory effect to MIP 1-bata and RANTES in neutrophil chemotaxis assay, indicating the antibody’s ability to neutralize IL-8 activity is specific. The antibody also neutralized IL-8 induced calcium influx in human granulocytes.

Western Blot: Concentration of 0.02-0.1μg/mL antibody will allow visualization of 100 ng/lane of human IL-8.

IHC-P: Used as an assay-dependent dilution.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G affinity purified
Buffer 0.01M PBS (pH 7.0)
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 3576 Human CXCL8

Swiss-port # P10145 Human Interleukin-8

Gene Symbol CXCL8
Gene Full Name chemokine (C-X-C motif) ligand 8
Background Interleukin 8 (IL-8), like IL-6, is secreted by macrophages and a variety of cells that express Toll-like receptors in response to the stimulation of pathogens. IL-8's primary function is to recruit neutrophils and other target cells through chemotaxis to the infected site to eliminate pathogens. IL-8 causes increased intracellular Ca2+, release of reactive oxygen species, and other physiological changes required for migration and phagocytosis. IL-8 is also known to promote angiogenesis.
Function IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. [UniProt]
Resrarch Area Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody
PTM Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
Citrullination at Arg-27 prevents proteolysis, and dampens tissue inflammation, it also enhances leukocytosis, possibly through impaired chemokine clearance from the blood circulation.

Images (2) Click the Picture to Zoom In

  • ARG10006 anti-IL8 antibody [I8-60] WB image

    Western Blot: Recombinant human IL-8 stained with anti-IL8 antibody [I8-60] (ARG10006)

  • ARG10006 anti-IL8 antibody [I8-60] IHC-P image

    Immunohistochemistry: Stratum corneum of psoriatic skin tissue stained with ARG10006 anti-IL8 antibody [I8-60] followed by incubation with Biotinylated secondary antibody and avidin-HRP. The specimen was stained with DAB substrate for 5~10 min, and counter-stained with hematoxylin.