ARG56624

anti-TNF alpha antibody

anti-TNF alpha antibody for ELISA,IHC-Frozen sections,Neutralizing,Western blot and Rat,Zebrafish

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Overview

Product Description Rabbit Polyclonal antibody recognizes TNF alpha
Tested Reactivity Rat, Zfsh
Tested Application ELISA, IHC-Fr, Neut, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TNF alpha
Antigen Species Rat
Immunogen E.coli derived Recombinant Rat TNF-α.
(MLRSSSQNSS DKPVAHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVFQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL)
Conjugation Un-conjugated
Alternate Names Tumor necrosis factor ligand superfamily member 2; DIF; Cachectin; ICD2; ICD1; N-terminal fragment; TNF-a; TNFA; TNFSF2; TNF-alpha; Tumor necrosis factor; NTF

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml as a capture antibody
IHC-Fr1.0 µg/ml
Neut0.07 - 0.10 µg/ml (To yield [ND50] of the biological activity of Rat TNF - α (2.0 ng/ml) )
WB0.1 - 0.2 µg/ml
Application Note Sandwich ELISA (Capture antibody - Detection antibody): ARG56624 - ARG43400

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 24835 Rat TNF

Swiss-port # P16599 Rat Tumor necrosis factor

Gene Symbol Tnf
Gene Full Name tumor necrosis factor
Background This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
Function Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective. Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line.

The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. [UniProt]
Highlight Related products:
TNF alpha antibodies; TNF alpha ELISA Kits; TNF alpha Duos / Panels; TNF alpha recombinant proteins; Anti-Rabbit IgG secondary antibodies;
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HMGB1 in inflammation
Inflammatory Cytokines
Calculated MW 26 kDa
PTM The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.

Images (3) Click the Picture to Zoom In

  • ARG56624 anti-TNF alpha antibody WB image

    Western blot: 250 - 0.24 ng of Rat TNF-α stained with ARG56624 anti-TNF alpha antibody, under reducing conditions.

  • ARG56624 anti-TNF alpha antibody WB image

    Western blot: 250 - 0.24 ng of Rat TNF-α stained with ARG56624 anti-TNF alpha antibody, under non-reducing conditions.

  • ARG56624 anti-TNF alpha antibody standard curve image

    Sandwich ELISA: ARG56624 anti-TNF alpha antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG43400 anti-TNF alpha antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.

Specific References

Intake of acrylamide at the dietary relevant concentration causes splenic toxicity in adult zebrafish.

IHC-Fr / Zebrafish

Yuta Komoike et al.
Environmental Research.,  (2020)

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