anti-TNF alpha antibody
anti-TNF alpha antibody for ELISA,IHC-Frozen sections,Neutralizing,Western blot and Rat,Zebrafish
|Product Description||Rabbit Polyclonal antibody recognizes TNF alpha|
|Tested Reactivity||Rat, Zfsh|
|Tested Application||ELISA, IHC-Fr, Neut, WB|
|Target Name||TNF alpha|
|Immunogen||E.coli derived Recombinant Rat TNF-α.
(MLRSSSQNSS DKPVAHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVFQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL)
|Alternate Names||Tumor necrosis factor ligand superfamily member 2; DIF; Cachectin; ICD2; ICD1; N-terminal fragment; TNF-a; TNFA; TNFSF2; TNF-alpha; Tumor necrosis factor; NTF|
|Application Note||Sandwich ELISA (Capture antibody - Detection antibody): ARG56624 - ARG43400
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Purification||Affinity purification with immunogen.|
|Buffer||PBS (pH 7.2)|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||tumor necrosis factor|
|Background||This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]|
|Function||Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective. Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line.
The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. [UniProt]
TNF alpha antibodies; TNF alpha ELISA Kits; TNF alpha Duos / Panels; TNF alpha recombinant proteins; Anti-Rabbit IgG secondary antibodies;
HMGB1 in inflammation
|Calculated MW||26 kDa|
|PTM||The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
Images (3) Click the Picture to Zoom In
ARG56624 anti-TNF alpha antibody WB image
Western blot: 250 - 0.24 ng of Rat TNF-α stained with ARG56624 anti-TNF alpha antibody, under reducing conditions.
ARG56624 anti-TNF alpha antibody WB image
Western blot: 250 - 0.24 ng of Rat TNF-α stained with ARG56624 anti-TNF alpha antibody, under non-reducing conditions.
ARG56624 anti-TNF alpha antibody standard curve image
Sandwich ELISA: ARG56624 anti-TNF alpha antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG43400 anti-TNF alpha antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.