ARG63621

anti-alpha smooth muscle Actin antibody

anti-alpha smooth muscle Actin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Pig,Rat

Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody
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anti-alpha smooth muscle Actin antibody

Goat Polyclonal antibody recognizes alpha smooth muscle Actin

ARG63621 Arigo biolaboratories
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anti-alpha smooth muscle Actin antibody

Overview

Product Description

Goat Polyclonal antibody recognizes alpha smooth muscle Actin

Tested Reactivity Hu, Ms, Rat, Pig
Predict Reactivity Dog
Tested Application ICC/IF, IHC-P, WB
Specificity Variants NP_001604.1 and NP_001135417.1 encode the same protein.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name alpha smooth muscle Actin
Antigen Species Human
Immunogen EEEDSTALVC
Conjugation Un-conjugated
Alternate Names Cell growth-inhibiting gene 46 protein; MYMY5; ACTSA; Alpha-actin-2; Actin, aortic smooth muscle; AAT6

Application Instructions

Application Note WB: Recommend incubate at RT for 1h. Approx 46 kDa band observed in Human Duodenum lysates (calculated MW of 42.0 kDa according to NP_001604.1). Recommended concentration: 0.5-1.5 µg/ml.
IHC-P: In paraffin embedded Human and Murine Lung shows staining of the alveolar walls. Recommended concentration, 2-4 µg/ml.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 42 kDa

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 11475 Mouse ACTA2

GeneID: 59 Human ACTA2

GeneID: 81633 Rat ACTA2

Background The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]
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Research Area Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody
PTM Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity).
Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.
(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).

Images (2) Click the Picture to Zoom In

  • ARG63621 anti-alpha smooth muscle Actin antibody WB image

    Western blot: 30 µg of MEF cell lysate stained with ARG63621 anti-alpha smooth muscle Actin antibody at 1:300 dilution.

  • ARG63621 anti-Smooth muscle alpha-actin antibody IHC-P image

    Immunohistochemistry: paraffin embedded Human Lung (top) and Mouse Lung (bottom). (Trypsin-mediated antigen retrieval) stained with ARG63621 anti-Smooth muscle alpha-actin antibody at 2.5 µg/ml dilution followed by HRP-staining.

Customer's Feedback

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Review for anti-alpha smooth muscle Actin antibody

Application:WB

Sample:MEF cell lysate

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:300

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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