Product Highlight

Product Highlight

arigo offers Antibody Duo and Antibody Panel products for researchers who work on various research topics. Please find arigo’s mini pathway figures featuring our high quality Duos and Panels for your experimental needs!

 

Apoptosis Pathway

In multicellular organisms, cells no longer needed or have become a threat to the organism will be destroyed by a tightly regulated suicide mechanism called programmed cell death, or more commonly known as Apoptosis.

Apoptosis process involves a cascade of proteolytic cleavage of Caspase molecules. Apoptosis can be initiated by intra- or extracellular death signals and the regulation of apoptosis can involve a wide variety of molecules.

arigo offers Antibody Duos for the study of key stages in Apoptosis regulation.

 

 

Product name: Apoptosis Marker Antibody Duo (Caspase 3, PARP)
Cat no: ARG30105

The TUNEL assay has been commonly used as a detection method for identifying apoptotic cells. While useful, it can also yield highly variable results as some non-apoptotic nuclei showing high levels of RNA synthesis/splicing can also be labeled by TUNEL technique.
Cleavage of Caspase 3 and PARP (Poly (ADP-ribose) Polymerase is a pre-requisite for cells who undergo final stage of cell death. The use of anti-cleaved caspase3 (ARG20003) and anti-cleaved PARP (ARG20041) antibodies adds a more specific weapon to the arsenal of techniques for confirming apoptosis in tissue specimens.

 

Product name: Mitochondrial Apoptosis Initiator Antibody Duo (APAF1, Cytochrome-C)
Cat no: ARG30107

Cytochrome C is an electron transport protein localized in the mitochondria. When a cell receive intra- or extra-cellular death signal, Cytochrome C is then released from mitochondria to cytoplasm and form an oligomeric apoptosome with APAF-1. The apoptosome binds to and cleave Procaspase 9, releasing its mature, activated form.
Detection of cytoplasmic Cytochrome C and APAF-1 colocalization in an apoptosome with anti-Cytochrome C (ARG62461, mouse mAB) and anti APAF-1 (ARG52529, rabbit pAB) antibodies are initiate indicators of cell death.

 

GSK3 Downstream Signaling Pathway

Constitutively active GSK-3 acts to destabilize beta catenin through phosphorylation (Ser37), thus, functioning as a tumor suppressor to limit proliferation. In contrast, GSK-3 promotes expansion of leukemic cells transformed by HOX overexpression through phosphorylation of CREB (Ser133) and subsequent formation of a transcriptional coactivator complex. (Cancer Cell 17, June 15, 2010)

arigo provides CREB / beta Catenin phospho Duos, ARG30024, which include beta catenin (phosphor Ser37) antibody and CREB (phosphor Ser133) antibody for researchers to analysis the downstream protein and the pathway of active-GSK-3

 

Product name: Phospho-CREB (pS133)/Phospho beta-Catenin (pS37) Antibody Duo
Cat no: ARG30024

 

Component:

ARG51556 CREB(pSer133)Antibody

WB: HeLa cells with or without UV treatment.       IHC: Human breast carcinoma tissue.
   

ARG51710 β Catenin (pSer37) Antibody

WB: HT29 cells with or without peptide blocking.       IHC: Human breast carcinoma tissue.

 

Embryonic Stem Cell Differentiation: Germ Layer Marker

Cat no: ARG30134

The germ layer is formed primarily during embryogenesis. Cells migrate inward along the archenteron to form the inner layer of the gastrula, which develops into the endoderm. The endoderm further derive to almost the whole digestive tube, including the lining cells of all the glands which open into the digestive tube, and the follicle lining of the thyroid gland and thymus. The mesoderm, one of the three germinal layers, consists of three important components, the paraxial mesoderm, the intermediate mesoderm and the lateral plate mesoderm. The mesoderm develops into the notochord, kidneys, gonads, cartilage, skeletal muscle, dermis, the circulatory system (including the heart and spleen), the wall of the gut, and wall of the human body. The ectoderm generates the outer layer of the embryo, and it forms from the embryo's epiblast. The ectoderm develops into the surface ectoderm, neural crest, and the neural tube.
This antibody panel investigates the formation of different germ layers during embryogenesis or iPS stimulation. Tuj1 (ectoderm marker), a-SMA (mesoderm marker) and AFP (endoderm marker) could be used to monitor the process of embryonic differentiation.

 

1. Katsetos, C.D. et al. (2003) J Child Neurol 18, 851-66; discussion 867.
2. Skalli, O. et al. (1989) J Histochem Cytochem 37: 315
3. Kwon, G.S. et al. (2006) Dev Dyn 235(9): 2549–2558.

 

Ectodermal Marker
TUJI (ARG52460)
Mesodermal Marker
SMA (ARG53568)
Endodermal Marker
AFP (ARG52509)

 

General Lymphocyte Marker Antibody Panel (CD3, CD14, CD16, CD19, CD56)

Cat no: ARG30143

arigo offers a wide range of markers for the study of lymphocytes. CD19 is expressed on follicular dendritic cells and B cells. CD3 is expressed on T cells and is responsible for T Cell activation. CD14 is expressed mainly by macrophages and neutrophils. CD16 and CD56 are found on the surface of natural killer cells.
Tjomsland, V. et al. (2010) PloS one 5, e13441
      NKT Marker
      CD16 (ARG53773)
      NKT Marker
      CD56 (ARG53879)
      Macrophage marker
      CD14 (ARG53766)

FACS: Human peripheral blood cells.

 
  B cell marker
CD19 (ARG52708)
  T Cell marker
CD3 (ARG52742)
 
 
 
 
      IHC: Human tonsil tissues.