ARG30321

Autophagy Antibody Panel

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG65614 anti-Beclin 1 antibody Rabbit pAb Hu, Ms, Rat IHC-P, WB 20 μl
ARG55799 anti-LC3B antibody Rabbit pAb Hu, Ms, Rat ICC/IF, IHC-P, WB 20 μl
ARG55040 anti-SQSTM1 / p62 antibody Rabbit pAb Hu, Ms ICC/IF, IHC-P, IP, WB 20 μl
ARG66330 anti-GAPDH antibody [SQab1878] Rabbit mAb AGMK, Bov, Chk, Hu, Ms, Pig, Rat, Xenopus laevis, Zfsh FACS, ICC/IF, IHC-P, IP, WB 20 μl
ARG65351 Goat anti-Rabbit IgG antibody (HRP) Goat pAb Rb ELISA, IHC-P, WB 50 μl

Properties

Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Highlight Related products:
Autophagy antibodies; Autophagy Duos / Panels;
Related news:
New Autophagy antibody panel is released

(21) Click the Picture to Zoom In

  • ARG65614 anti-Beclin 1 antibody WB image

    Western blot: 30 µg of A549, U87-MG, HepG2, Rat brain, and Rat ovary lysates stained with ARG65614 anti-Beclin 1 antibody at 1:500 dilution.

  • ARG55799 anti-LC3B antibody WB image

    Western blot: HeLa cells untreated or treated with Chloroquine. 20 µg of cell lysates stained with ARG55040 anti-SQSTM1 / p62 antibody and ARG55799 anti-LC3B antibody at 1:1000 dilution.

    An anti-GAPDH antibody used as a positive control.

  • ARG55040 anti-SQSTM1 / p62 antibody ICC/IF image

    Immunofluorescence: U2OS cells stained with ARG55040 anti-SQSTM1 / p62 antibody at 1:100 dilution.

  • ARG55799 anti-LC3B antibody ICC/IF image

    Immunofluorescence: U20S cell stained with ARG55799 anti-LC3B antibody at 1:100 dilution.

  • ARG66330 anti-GAPDH antibody [SQab1878] ICC/IF image

    Immunofluorescence: HeLa cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66330 anti-GAPDH antibody [SQab1878] (red) at 1:50,000 and 4°C. DAPI (blue) was used as the nuclear counter stain.

  • ARG66330 anti-GAPDH antibody [SQab1878] FACS image

    Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The were stained with ARG66330 anti-GAPDH antibody [SQab1878] (red) at 1:1,000 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control. .

  • ARG65614 anti-Beclin 1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human liver cancer tissue stained with ARG65614 anti-Beclin 1 antibody (left) at 1/20 dilution, or the same antibody preincubated with antigen (right). (Original magnification: X200).

  • ARG55040 anti-SQSTM1 / p62 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat brain stained with ARG55040 anti-SQSTM1 / p62 antibody at 1:100 dilution.

  • ARG55799 anti-LC3B antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat kidney stained with ARG55799 anti-LC3B antibody at 1:100 dilution.

  • ARG66330 anti-GAPDH antibody [SQab1878] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded prostate cancer tissue stained with ARG66330 anti-GAPDH antibody [SQab1878] at 1:1,600 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.

  • ARG65614 anti-Beclin 1 antibody WB image

    Western blot: 40 µg of NIH/3T3 cell lysate stained with ARG65614 anti-Beclin 1 antibody at 1:700 dilution.

  • ARG66330 anti-GAPDH antibody [SQab1878] WB image

    Western blot: 2 µg of A431, MCF7, HCT-116, 293, HeLa, Raji and Jurkat cell lysates stained with ARG66330 anti-GAPDH antibody [SQab1878] at 1:40000 dilution.

  • ARG66330 anti-GAPDH antibody [SQab1878] IP image

    Immunoprecipitation: 0.1 mg of HeLa whole cell lysate immunoprecipitated (1:50) and stained with ARG66330 anti-GAPDH antibody [SQab1878]. 1) ARG66330 IP in HeLa whole cell lysate, 2) PBS instead of ARG66330 in HeLa whole cell lysate, and 3) HeLa whole cell lysate, 2 µg (input).

  • ARG65614 anti-Beclin 1 antibody WB image

    Western blot: 20 µg of HeLa cell lysate stained with ARG65614 anti-Beclin 1 antibody at 1:500 dilution.

  • ARG55040 anti-SQSTM1 / p62 antibody WB image

    Western blot: HeLa cells untreated or treated with Chloroquine. 20 µg of cell lysates stained with ARG55040 anti-SQSTM1 / p62 antibody at 1:1000 dilution.

  • ARG55799 anti-LC3B antibody WB image

    Western blot: 30 µg of HeLa untreated or treated with Chloroquine at 50 µM, 100 µM (18 hr). The bolts stained with ARG55799 anti-LC3B antibody at 1:1000 dilution.

  • ARG55040 anti-SQSTM1 / p62 antibody WB image

    Western blot: 30 µg of HeLa and A549 cell lysates stained with ARG55040 anti-SQSTM1 / p62 antibody at 1:500 dilution.

  • ARG65614 anti-Beclin 1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human colon cancer tissue stained with ARG65614 anti-Beclin 1 antibody (left) at 1/70 dilution, or the same antibody preincubated with antigen (right). (Original magnification: X200).

  • ARG66330 anti-GAPDH antibody [SQab1878] WB image

    Western blot: 2 µg of Mouse brain, Mouse heart, Mouse kidney, Mouse liver, Rat brain, Rat heart, Rat kidney and Rat liver lysates stained with ARG66330 anti-GAPDH antibody [SQab1878] at 1:40000 dilution.

  • ARG66330 anti-GAPDH antibody [SQab1878] WB image

    Western blot: 2 µg of MDBK, Cos-7, Chicken heart, Pig heart, African clawed frog and Zebrafish lysates stained with ARG66330 anti-GAPDH antibody [SQab1878] at 1:40000 dilution.

  • ARG55799 anti-LC3B antibody WB image

    Western blot: 30 µg of SW620, Mouse spleen, and Rat spleen lysates stained with ARG55799 anti-LC3B antibody at 1:1000 dilution.