ARG30321

Autophagy Antibody Panel

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG65614 anti-Beclin 1 antibody Rabbit pAb Hu, Ms, Rat IHC-P, WB 20 μl
ARG55799 anti-LC3B antibody Rabbit pAb Hu, Ms, Pig, Rat ICC/IF, IHC-P, WB 20 μl
ARG55040 anti-SQSTM1 / p62 antibody Rabbit pAb Hu, Ms ICC/IF, IHC-P, IP, WB 20 μl
ARG66330 anti-GAPDH antibody [SQab1878] Rabbit mAb AGMK, Bov, Chk, Hu, Ms, Pig, Rat, Xenopus laevis, Zfsh FACS, ICC/IF, IHC-P, IP, WB 20 μl
ARG65351 Goat anti-Rabbit IgG antibody (HRP) Goat pAb Rb ELISA, IHC-P, WB 50 μl

Overview

Product Description Autophagy Antibody Panel is an all-in-one solution to make autophagy research easy and economic. This antibody panel comprises antibodies against autophagy markers LC3B and SQSTM1 and antibody against the key regulator Beclin-1. It is ideal for not only autophagy detection but also investigation of autophagy regulation. Moreover, the most suitable loading control GAPDH antibody and the compatible secondary antibody are included in this panel. All the antibodies in this panel have excellent performance for multiple applications on multiple species.

Properties

Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Highlight Related products:
Autophagy antibodies; Autophagy Duos / Panels;
Related news:
New Autophagy antibody panel is released

Images (21) Click the Picture to Zoom In

  • ARG65614 anti-Beclin 1 antibody WB image

    Western blot: 30 µg of A549, U87-MG, HepG2, Rat brain, and Rat ovary lysates stained with ARG65614 anti-Beclin 1 antibody at 1:500 dilution.

  • ARG55799 anti-LC3B antibody WB image

    Western blot: HeLa cells untreated or treated with Chloroquine. 20 µg of cell lysates stained with ARG55040 anti-SQSTM1 / p62 antibody and ARG55799 anti-LC3B antibody at 1:1000 dilution.

    An anti-GAPDH antibody used as a positive control.

  • ARG55040 anti-SQSTM1 / p62 antibody ICC/IF image

    Immunofluorescence: U2OS cells stained with ARG55040 anti-SQSTM1 / p62 antibody at 1:100 dilution.

  • ARG55799 anti-LC3B antibody ICC/IF image

    Immunofluorescence: U20S cell stained with ARG55799 anti-LC3B antibody at 1:100 dilution.

  • ARG66330 anti-GAPDH antibody [SQab1878] ICC/IF image

    Immunofluorescence: HeLa cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66330 anti-GAPDH antibody [SQab1878] (red) at 1:50,000 and 4°C. DAPI (blue) was used as the nuclear counter stain.

  • ARG66330 anti-GAPDH antibody [SQab1878] FACS image

    Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The were stained with ARG66330 anti-GAPDH antibody [SQab1878] (red) at 1:1,000 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control. .

  • ARG65614 anti-Beclin 1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human liver cancer tissue stained with ARG65614 anti-Beclin 1 antibody (left) at 1/20 dilution, or the same antibody preincubated with antigen (right). (Original magnification: X200).

  • ARG55040 anti-SQSTM1 / p62 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat brain stained with ARG55040 anti-SQSTM1 / p62 antibody at 1:100 dilution.

  • ARG55799 anti-LC3B antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat kidney stained with ARG55799 anti-LC3B antibody at 1:100 dilution.

  • ARG66330 anti-GAPDH antibody [SQab1878] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded prostate cancer tissue stained with ARG66330 anti-GAPDH antibody [SQab1878] at 1:1,600 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.

  • ARG65614 anti-Beclin 1 antibody WB image

    Western blot: 40 µg of NIH/3T3 cell lysate stained with ARG65614 anti-Beclin 1 antibody at 1:700 dilution.

  • ARG66330 anti-GAPDH antibody [SQab1878] WB image

    Western blot: 2 µg of A431, MCF7, HCT-116, 293, HeLa, Raji and Jurkat cell lysates stained with ARG66330 anti-GAPDH antibody [SQab1878] at 1:40000 dilution.

  • ARG66330 anti-GAPDH antibody [SQab1878] IP image

    Immunoprecipitation: 0.1 mg of HeLa whole cell lysate immunoprecipitated (1:50) and stained with ARG66330 anti-GAPDH antibody [SQab1878]. 1) ARG66330 IP in HeLa whole cell lysate, 2) PBS instead of ARG66330 in HeLa whole cell lysate, and 3) HeLa whole cell lysate, 2 µg (input).

  • ARG65614 anti-Beclin 1 antibody WB image

    Western blot: 20 µg of HeLa cell lysate stained with ARG65614 anti-Beclin 1 antibody at 1:500 dilution.

  • ARG55040 anti-SQSTM1 / p62 antibody WB image

    Western blot: HeLa cells untreated or treated with Chloroquine. 20 µg of cell lysates stained with ARG55040 anti-SQSTM1 / p62 antibody at 1:1000 dilution.

  • ARG55799 anti-LC3B antibody WB image

    Western blot: 30 µg of HeLa untreated or treated with Chloroquine at 50 µM, 100 µM (18 hr). The bolts stained with ARG55799 anti-LC3B antibody at 1:1000 dilution.

  • ARG55040 anti-SQSTM1 / p62 antibody WB image

    Western blot: 30 µg of HeLa and A549 cell lysates stained with ARG55040 anti-SQSTM1 / p62 antibody at 1:500 dilution.

  • ARG65614 anti-Beclin 1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human colon cancer tissue stained with ARG65614 anti-Beclin 1 antibody (left) at 1/70 dilution, or the same antibody preincubated with antigen (right). (Original magnification: X200).

  • ARG66330 anti-GAPDH antibody [SQab1878] WB image

    Western blot: 2 µg of Mouse brain, Mouse heart, Mouse kidney, Mouse liver, Rat brain, Rat heart, Rat kidney and Rat liver lysates stained with ARG66330 anti-GAPDH antibody [SQab1878] at 1:40000 dilution.

  • ARG66330 anti-GAPDH antibody [SQab1878] WB image

    Western blot: 2 µg of MDBK, Cos-7, Chicken heart, Pig heart, African clawed frog and Zebrafish lysates stained with ARG66330 anti-GAPDH antibody [SQab1878] at 1:40000 dilution.

  • ARG55799 anti-LC3B antibody WB image

    Western blot: 30 µg of SW620, Mouse spleen, and Rat spleen lysates stained with ARG55799 anti-LC3B antibody at 1:1000 dilution.