ARG30139

Cell Cycle Marker Antibody Panel (Cyclin B1, Cyclin E)

Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody
publication_link Publication3

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG52927 anti-Cyclin E1 antibody Rabbit pAb Hu IHC-P 250 μl
ARG62456 anti-Cyclin B1 antibody [V152] Mouse mAb Hu FACS, ICC/IF, IHC-P, IHC-Fr, WB 250 μl
ARG51679 anti-Histone H3 phospho (Ser10) antibody Rabbit pAb Cydippids, Hu, Ms, Rat ICC/IF, IHC-P, WB 50 μl
ARG65350 Goat anti-Mouse IgG antibody (HRP) Goat pAb Ms ELISA, IHC-P, WB 50 μl
ARG65351 Goat anti-Rabbit IgG antibody (HRP) Goat pAb Rb ELISA, IHC-P, WB 50 μl

Overview

Product Description

The cell cycle is controlled by numerous mechanisms ensuring correct cell division. Cell division consists of two consecutive processes, mainly characterized by DNA replication (S phase), proceeding after G1 interphase; and segregation of replicated chromosomes into two separate cells (M phase), following G2 phase. Cyclin E is upregulated during G1/S progression whereas Cyclin A and Cyclin B are expressed highly during G2/M progression. Phosphorylated Histone H3 is a distinct marker for Mitosis and often used in determining cells undergoing M-phase in a proliferating populations.

Arigo’s Cell Cycle Phase Marker Panel provides a useful tool as markers for cells undergoing different phases in cell cycle.

Vermeulen et al. (2003) Cell Prolif 36:131-149
Rahman et al. (2010) Cell Cycle 9: 22-27
Hans and Dimitrov (2001) Oncogene 24: 3021-3027

Target Name Cell Cycle Marker
Alternate Names Cell Cycle Marker antibody; Histone H3 phospho (Ser10) antibody; Cyclin E1 antibody; Cyclin B1 antibody

Properties

Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Full Name Antibody Panel for Cell Cycle Marker (Cyclin B1, Cyclin E)
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody

Images (7) Click the Picture to Zoom In

  • ARG62456 anti-Cyclin B1 antibody [V152] WB image

    Western blot: 20 µl of HeLa cell lysate stained with ARG62456 anti-Cyclin B1 antibody [V152] at 1 µg/ml dilution.

  • ARG51679 anti-Histone H3 phospho (Ser10) antibody ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG51679 anti-Histone H3 phospho (Ser10) antibody at 1:100 dilution. Left: primary antibody (orange). Middle: DAPI (blue). Right: Merge.

    Secondary antibody: ARG21917 Goat anti-Rabbit IgG antibody (TRITC).

  • ARG51679 anti-Histone H3.1 phospho (Ser10) antibody WB image

    Western Blot: extracts from SK-BR-3 cells, treated with insulin and EGF, and pretreated with U0126 and LY294002 cells stained with anti-Histone H3.1 (phospho Ser10) antibody ARG51679.

  • ARG51679 anti-Histone H3.1 phospho (Ser10) antibody ICC/IF image

    Immunofluorescence: methanol-fixed HeLa cells stained with anti-Histone H3.1 (phospho Ser10) antibody ARG51679.

  • ARG51679 anti-Histone H3.1 phospho (Ser10) antibody IHC-P image

    Immunohistochemistry: paraffin-embedded human breast carcinoma tissue stained with anti-Histone H3.1 (phospho Ser10) antibody ARG51679 (left) or the same antibody preincubated with blocking peptide (right).

  • ARG51679 anti-Histone H3.1 phospho (Ser10) antibody ICC/IF image

    Immunofluorescence: methanol-fixed HeLa cells stained with anti-Histone H3.1 (phospho Ser10) antibody ARG51679.

  • ARG52927 anti-Cyclin E antibody IHC-P image

    Immunohistochemistry: Human Breast Carcinoma stained with Cyclin E antibody (ARG52927)

Specific References

GNL3 is an evolutionarily-conserved stem cell gene influencing cell proliferation, animal growth, and regeneration in the hydrozoan Hydractinia

ARG51679: ICC/IF / Hydractinia

Gonzalo Quiroga-Artigas et al.
biorxiv,  (2022)

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Silencing of AURKA augments the antitumor efficacy of the AURKA inhibitor MLN8237 on neuroblastoma cells.

ARG51679: IHC-P / Human

Yang Yan et al.
Cancer Cell Int.,  (2020)

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Scarless whole-body regeneration in the absence of a blastema requires cell division in the ctenophore Mnemiopsis leidyi

ARG51679: ICC/IF / Cydippids

Julia Ramon Mateu et al.
Preprint.,  (2019)

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