ARG30320

EMT Marker Antibody Panel

publication_link Publication7

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG66195 anti-E Cadherin antibody [SQab1717] Rabbit mAb Hu IHC-P, WB 20 μl
ARG66199 anti-Vimentin antibody [SQab1721] Rabbit mAb Hu, Ms FACS, ICC/IF, IHC-P, IP, WB 20 μl
ARG23870 anti-N Cadherin antibody Rabbit pAb Hu, Ms, Rat, Sheep ELISA, FACS, ICC/IF, IHC-P, IP, WB 20 μl
ARG66330 anti-GAPDH antibody [SQab1878] Rabbit mAb AGMK, Bov, Chk, Hu, Ms, Pig, Rat, Xenopus laevis, Zfsh FACS, ICC/IF, IHC-P, IP, WB 20 μl
ARG65351 Goat anti-Rabbit IgG antibody (HRP) Goat pAb Rb ELISA, IHC-P, WB 50 μl

Overview

Product Description EMT Marker Antibody Panel is an all-in-one solution to make EMT research easy and economic. This antibody panel comprises the antibodies against key epithelial marker E-Cadherin and key mesenchymal markers Vimentin and N-Cadherin. Moreover, the most suitable loading control GAPDH antibody and the compatible secondary antibody are included in this panel. All the antibodies in this panel have excellent performance for not only WB but also IHC-P and more applications.

Related news:
New EMT antibody panel is released
Target Name EMT Marker
Alternate Names EMT Marker antibody; N Cadherin antibody; E Cadherin antibody; Vimentin antibody; GAPDH antibody

Properties

Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (21) Click the Picture to Zoom In

  • ARG66195 anti-E Cadherin antibody [SQab1717] WB image

    Western blot: 20 µg of MCF7 cell lysate stained with ARG66195 anti-E Cadherin antibody [SQab1717] at 1:2000 dilution.

  • ARG66199 anti-Vimentin antibody [SQab1721] WB image

    Western blot: 20 µg of HeLa and 293T cell lysates stained with ARG66199 anti-Vimentin antibody [SQab1721] at 1:1000 dilution.

  • ARG23870 anti-N Cadherin antibody WB image

    Western blot: 20 µg of mouse brain stained with ARG23870 anti-N Cadherin antibody at 1:1000 dilution. (Observed MW: ~130 kDa)

  • ARG66199 anti-Vimentin antibody [SQab1721] ICC/IF image

    Immunofluorescence: HeLa cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at room temperature. Cells were stained with ARG66199 anti-Vimentin antibody [SQab1721] (green) at 1:25000 and 4°C. DAPI (blue) was used as the nuclear counter stain.

  • ARG66330 anti-GAPDH antibody [SQab1878] ICC/IF image

    Immunofluorescence: HeLa cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66330 anti-GAPDH antibody [SQab1878] (red) at 4°C. DAPI (blue) was used as the nuclear counter stain.

  • ARG23870 anti-N Cadherin antibody ICC/IF image

    Immunofluorescence: MCF7-ADR cells stained with ARG23870 anti-N Cadherin antibody.

  • ARG66199 anti-Vimentin antibody [SQab1721] FACS image

    Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then stained with ARG66199 anti-Vimentin antibody [SQab1721] (red) at 1:500 dilution in 1x PBS/1% BSA for 30 min at 4°C, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.

  • ARG66330 anti-GAPDH antibody [SQab1878] FACS image

    Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The were stained with ARG66330 anti-GAPDH antibody [SQab1878] (red) in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control. .

  • ARG66195 anti-E Cadherin antibody [SQab1717] IHC-P image

    Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human skin tissue stained with ARG66195 anti-E Cadherin antibody [SQab1717] at 1:50 dilution.

    Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0

  • ARG66199 anti-Vimentin antibody [SQab1721] IHC-P image

    Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human colon tissue stained with ARG66199 anti-Vimentin antibody [SQab1721] at 1:1000 dilution.

    Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0

  • ARG66330 anti-GAPDH antibody [SQab1878] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded prostate cancer tissue stained with ARG66330 anti-GAPDH antibody [SQab1878]. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.

  • ARG23870 anti-N Cadherin antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded human pancreatic adenocarcinoma stained with ARG23870 anti-N Cadherin antibody.

  • ARG66330 anti-GAPDH antibody [SQab1878] WB image

    Western blot: 2 µg of A431, MCF7, HCT-116, 293, HeLa, Raji and Jurkat cell lysates stained with ARG66330 anti-GAPDH antibody [SQab1878].

  • ARG23870 anti-N Cadherin antibody WB image

    Western blot: Human endothelial cells untreated (left) or treated with Pervanadate (1 mM) for 30 min (right). The blots were stained with ARG23870 anti-N Cadherin antibody. (Observed MW: ~130 kDa)

  • ARG66199 anti-Vimentin antibody [SQab1721] IP image

    Immunoprecipitation: 0.4 mg of HeLa cell lysate immunoprecipitated and stained with ARG66199 anti-Vimentin antibody [SQab1721]. 1) IP in HeLa whole cell lysate, 2) Rabbit IgG instead of primary antibody in HeLa whole cell lysate and 3) HeLa whole cell lysate, 10 μg (input).

  • ARG66330 anti-GAPDH antibody [SQab1878] IP image

    Immunoprecipitation: 0.1 mg of HeLa whole cell lysate immunoprecipitated and stained with ARG66330 anti-GAPDH antibody [SQab1878]. 1) ARG66330 IP in HeLa whole cell lysate, 2) PBS instead of ARG66330 in HeLa whole cell lysate, and 3) HeLa whole cell lysate, 2 µg (input).

  • ARG23870 anti-N Cadherin antibody IP image

    Immunoprecipitation: Mouse brain lysate immunoprecipitated with no antibody (lane 1), ARG23870 anti-N Cadherin antibody (lane 2) and whole mouse brain lysate (lane 3). The blot was stained with anti-N Cadherin (Cytoplasmic) Mouse monoclonal antibody (lanes 1-3).

  • ARG66199 anti-Vimentin antibody [SQab1721] WB image

    Western blot: 20 µg of COLO205, HCT116, HT29 (Vimentin unexpression cell lines) and SW620 (Vimentin expression cell line). Cell lysates stained with ARG66199 anti-Vimentin antibody [SQab1721] at 1:1000 dilution.

  • ARG66195 anti-E Cadherin antibody [SQab1717] IHC-P image

    Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human gastric adenocarcinoma tissue stained with ARG66195 anti-E Cadherin antibody [SQab1717] at 1:50 dilution.

    Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0

  • ARG66330 anti-GAPDH antibody [SQab1878] WB image

    Western blot: 2 µg of Mouse brain, Mouse heart, Mouse kidney, Mouse liver, Rat brain, Rat heart, Rat kidney and Rat liver lysates stained with ARG66330 anti-GAPDH antibody [SQab1878].

  • ARG66330 anti-GAPDH antibody [SQab1878] WB image

    Western blot: 2 µg of MDBK, Cos-7, Chicken heart, Pig heart, African clawed frog and Zebrafish lysates stained with ARG66330 anti-GAPDH antibody [SQab1878].

Specific References

Necroptotic virotherapy of oncolytic alphavirus M1 cooperated with Doxorubicin displays promising therapeutic efficacy in TNBC

ARG66330: WB / Human

Jiayu Zhang et al.
Oncogene,  (2021)

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hr_line

OC-STAMP Overexpression Drives Lung Alveolar Epithelial Cell Type II Senescence in Silicosis.

ARG23870: WB / Rat

Tian Li et al.
Oxidative Medicine and Cellular Longevity.,  (2021)

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hr_line

A novel mechanism driving poor-prognostic gastric cancer: overexpression of the transcription factor Krüppel-like factor 16 promotes growth and metastasis of gastric cancer through regulating the Notch pathway.

ARG66195: WB / Human

Ding-Ping Sun et al.
Am J Cancer Res.,  (2021)

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hr_line

Ubiquitin specific peptidase 5 enhances STAT3 signaling and promotes migration and invasion in Pancreatic Cancer.

ARG66199: WB / Human

Jie Lian et al.
J Cancer.,  (2020)

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hr_line

ACE2 Attenuates Epithelial-Mesenchymal Transition in MLE-12 Cells Induced by Silica.

ARG66199: WB / Mouse

Li Shumin et al.
Drug Des Devel Ther.,  (2020)

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hr_line

A super-enhancer controls TGF- β signaling in pancreatic cancer through downregulation of TGFBR2.

ARG66199: WB / Human

Zhu Xiaolin et al.
Cell Signal.,  (2019)

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hr_line

Tumor-associated neutrophils induce EMT by IL-17a to promote migration and invasion in gastric cancer cells.

ARG66195, ARG66199: WB / Human

Li Sen et al.
J Exp Clin Cancer Res.,  (2019)

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