ARG80111

Human IL8 ELISA Kit

Human IL8 ELISA Kit for ELISA and Human

Cancer kit; Cell Biology and Cellular Response kit; Immune System kit
publication_link Publications2

Component

Cat No Component Name Package Temp
ARG80111-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80111-002 Standard (Lyophilized) 3 X 1 ng/vial 4°C
ARG80111-003 Standard diluent buffer 20 ml 4°C
ARG80111-004 Antibody conjugate concentrate 400 µl 4°C
ARG80111-005 Antibody diluent buffer 16 ml 4°C
ARG80111-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80111-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80111-008 20X Wash buffer 50 ml 4°C
ARG80111-009 TMB substrate 12ml 4°C (Protect from light)
ARG80111-010 STOP solution 12ml 4°C
ARG80111-011 Plate sealer 6 strips Room temperature

Overview

Product Description ARG80111 Human IL8 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IL8 in Serum, Plasma, Cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human GRO alpha, GRO beta, GRO gamma, I-309, IP-10, MCP-1, MCP-2, MCP-3, MIP-1 alpha, MIP-1 beta, RANTES;mouse KC
Target Name IL8
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 4 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 7.8 - 500 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Full Name chemokine (C-X-C motif) ligand 8
Alternate Names IL8/NAP1 form IV; GCP/IL-8 protein IV; NAF; T-cell chemotactic factor; 1-77; Ala-IL-8; Interleukin-8; IL-8; Neutrophil-activating protein 1; GCP/IL-8 protein II; IL8/NAP1 form II; GCP/IL-8 protein V; MDNCF; Protein 3-10C; Lymphocyte-derived neutrophil-activating factor; Neutrophil-activating factor; Granulocyte chemotactic protein 1; LYNAP; NAP-1; Monocyte-derived neutrophil chemotactic factor; 6-77; 7-77; C-X-C motif chemokine 8; GCP1; NAP1; Ser-IL-8; 5-77; GCP/IL-8 protein VI; IL8/NAP1 form I; IL8/NAP1 form VI; Monocyte-derived neutrophil-activating peptide; C-X-C motif; 8-77; 9-77; LUCT; Chemokine; GCP-1; MDNCF-b; MDNCF-c; IL8/NAP1 form V; LECT; IL8/NAP1 form III; GCP/IL-8 protein III; Emoctakin; GCP/IL-8 protein I; MONAP; IL8

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol CXCL8
Gene Full Name chemokine (C-X-C motif) ligand 8
Background The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. [provided by RefSeq, Jul 2008]
Function IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. [UniProt]
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Research Area Cancer kit; Cell Biology and Cellular Response kit; Immune System kit
PTM Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
Citrullination at Arg-27 prevents proteolysis, and dampens tissue inflammation, it also enhances leukocytosis, possibly through impaired chemokine clearance from the blood circulation.

Images (1) Click the Picture to Zoom In

  • ARG80111 Human IL8 ELISA Kit standard curve image

    ARG80111 Human IL8 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Specific References

The Helicobacter pylori J99 jhp0106 Gene, under the Control of the CsrA/RpoN Regulatory System, Modulates Flagella Formation and Motility.

ELISA / Huamn

Kao CY et al.
Front Microbiol.,  (2017)

publication_link

 

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Implantable biomedical sensor array with biocompatible hermetic encapsulation

ELISA / Human

Carola Jorsch et al.
J. Sens. Sens. Syst.,  (2016)

publication_link

 

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Title Download Link
ARG80111 Human IL8 ELISA Kit User's manual download icon Download