ARG30267

Organelle Marker, Cytoplasm, Nucleus Antibody Duo (Histone, GAPDH)

publication_link Publication9

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG65681 anti-Histone H3 antibody Mouse mAb Hu, Ms, Rat ICC/IF, IHC-P, IP, WB 50 μg
ARG10112 anti-GAPDH antibody [6C5] Mouse mAb AGMK, Bb, Cat, Chk, Dog, Fsh, Hm, Hu, Mk, Ms, Pig, Rb, Rat, Xenopus laevis, Zfsh ELISA, ICC/IF, IHC-Fr, WB 50 μg

Overview

Product Description

GAPDH is often stably and constitutively expressed at high levels in most tissues and cells in cytoplasm. Therefore, GAPDH is a widely used loading control and cytoplasm marker for western blotting (35-40kDa) and Immunostaining study.

Histone H3 (15-20kDa) is one of the component of nucleosomes in nucleus. So Histone H3 is one of the commonly used loading control and nucleus marker in western blotting and Immunostaining study.

This Cytoplasm, Nucleus antibody Duos not only provide different MW targets for internal control selection, but also provide cytoplasm (GAPDH) and nucleus (Histone H3) markers to control the quality of cytoplasm and nucleus fractions after subcellular fractionation process and to identify the cytoplasm and nucleus position in Immunostaining study.

Target Name Organelle Marker, Cytoplasm, Nucleus
Alternate Names Organelle Marker, Cytoplasm, Nucleus antibody; GAPDH antibody; Histone H3 antibody

Properties

Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (15) Click the Picture to Zoom In

  • ARG10112 anti-GAPDH antibody [6C5] WB image

    Western blot: 1) U87-MG 2) HepG2 3) rat brain 4) rat heart 5) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:2000 dilution.

  • ARG10112 anti-GAPDH antibody [6C5] ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG10112 anti-GAPDH antibody [6C5] (green) at 1:200 dilution.

    Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)

  • ARG65681 anti-Histone H3 antibody ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG65681 anti-Histone H3 antibody at 1:100 dilution. Left: primary antibody (green). Right: Merge (primary antibody and DAPI).

    Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)

  • ARG65681 anti-Histone H3 antibody WB image

    Western blot: 20 µg of HeLa, Mouse brain and Rat brain lysates stained with ARG65681 anti-Histone H3 antibody at 1:2000 dilution.

  • ARG65681 anti-Histone H3 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat testis tissue stained with ARG65681 anti-Histone H3 antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min. Secondary antibody was diluted at 1:200 (RT, 30 min).

    Negative control was used by secondary antibody only.

  • ARG65681 anti-Histone H3 antibody IP image

    Immunoprecipitation: 1) HeLa cell lysate stained with ARG65681 anti-Histone H3 antibody and 2) IP product immunoprecipitated by ARG65681 anti-Histone H3 antibody at 1:200 dilution.

  • ARG65681 anti-Histone H3 antibody WB image

    Western blot: 1) HeLa, 2) Raw, 3) Mouse brain tissue, and 4) Rat brain tissue lysates stained with ARG65681 anti-Histone H3 antibody at 1:5000 dilution.

  • ARG65681 anti-Histone H3 antibody IP image

    Immunoprecipitation: 1) HeLa cell lysate stained with ARG65681 anti-Histone H3 antibody and 2) IP product immunoprecipitated by ARG65681 anti-Histone H3 antibody at 1:200 dilution.

  • ARG10112 anti-GAPDH antibody [6C5] WB image

    Western blot: 1) MCF-7 2) DU-145 3) A549 4) H1299 5) HCT116 6) HepG2 7) HUVEC stained with ARG10112 anti-GAPDH antibody [6C5] at 1:1000 dilution.

  • ARG10112 anti-GAPDH antibody [6C5] WB image

    Western blot: 1) U87-MG 2) MCF-7 3) A549 4) DU145 5) SW480 6) rat brain 7) rat stomach 8) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:5000 dilution.

  • ARG10112 anti-GAPDH antibody [6C5] WB image

    Western blot: 1) U87-MG 2) MCF-7 3) A549 4) DU145 5) SW480 6) rat brain 7) rat stomach 8) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:5000 dilution.

  • ARG10112 anti-GAPDH antibody [6C5] WB image

    Western blot: 1) U87-MG 2) HepG2 3) rat brain 4) rat heart 5) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:2000 dilution.

  • ARG10112 anti-GAPDH antibody [6C5] WB image

    Western Blot: 1) HeLa, 2) NTERA-2, 3) A-431, 4) HepG2, 5) MCF-7, 6) NIH 3T3, 7) PC-12 and 8) COS-7 whole cell lysates stained with anti-GAPDH antibody [6C5] (ARG10112)

  • ARG10112 anti-GAPDH antibody [6C5] IP image

    Immunoprecipitation and western blot: 1) GAPDH (1 μg). 2) GAPDH IP from rat heart tissue extract. 3) Only GAPDH preincubated with Protein A Sepharose. 4) Only Protein A Sepharose stained with ARG10112 GAPDH antibody [6C5].


    Mixture of protein A-Sepharose with ARG10112 anti-GAPDH and tissue extract was incubated for 30 min at room temperature and precipitated by centrifugation. Pellet was washed with PBS, suspended in reducing electrophoresis sample buffer and heated for 5 minutes at 100ºC. After centrifugation supernatant was loaded on gel and proteins were separated by SDS electrophoresis.

  • ARG10112 anti-GAPDH antibody [6C5] WB image

    Western blot: 1) MCF-7 2) DU-145 3) A549 4) H1299 5) HCT116 6) HepG2 7) HUVEC stained with ARG10112 anti-GAPDH antibody [6C5] at 1:1000 dilution.

Specific References

Curcumin attenuates renal ischemia reperfusion injury via JNK pathway with the involvement of p300/CBP-mediated histone acetylation

ARG10112: WB / Rat

Lu Yang et al.
Korean J Physiol Pharmacol.,  (2021)

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7,8-dihydroxyflavone ameliorates motor deficits via regulating autophagy in MPTP-induced mouse model of Parkinson's disease

ARG10112: WB / Mouse

Li Zuo et al.
Cell Death Discov.,  (2021)

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Evaluation of Class IIa Histone Deacetylases Expression and In Vivo Epigenetic Imaging in a Transgenic Mouse Model of Alzheimer's Disease.

ARG10112: WB / Human

Yi-An Chen et al.
International Journal of Molecular Sciences.,  (2021)

publication_link

 

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Cardiac-Specific Gene TNNI3 as a Potential Oncogene for Kidney Cancer and Its Involvement in Wnt Signaling Pathway.

ARG10112: WB / Human

Weian Zhao et al.
Preprint.,  (2021)

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Mesenchymal Stem Cell-Derived Exosomes Ameliorate Alzheimer's Disease Pathology and Improve Cognitive Deficits.

ARG10112: WB / Human

Yi-An Chen et al.
Biomedicines.,  (2021)

publication_link

 

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Circ_0081572 inhibits the progression of periodontitis through regulating the miR-378h/RORA axis.

ARG10112: WB / Human

Jing Wang et al.
Arch Oral Biol.,  (2021)

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Structural Basis of VSIG3: The Ligand for VISTA.

ARG10112: WB / Human

Xiaoxue Xie et al.
Front Immunol.,  (2021)

publication_link

 

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The Phosphorylation Status of Drp1-Ser637 by PKA in Mitochondrial Fission Modulates Mitophagy via PINK1/Parkin to Exert Multipolar Spindles Assembly during Mitosis.

ARG10112: WB / Human

Huey-Jiun Ko et al.
Biomolecules.,  (2021)

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M351-0056 is a novel low MW compound modulating the actions of the immune-checkpoint protein VISTA.

ARG10112: WB / Human, Mouse

Xinlei Hu et al.
Br J Pharmacol.,  (2021)

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