ARG30330

Pyroptosis Antibody Panel

publication_link Publication4

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG57293 anti-Caspase 1 antibody Rabbit pAb Hu, Ms ICC/IF, IHC-P, IP, WB 20 μl
ARG41404 anti-GSDMD antibody Rabbit pAb Hu, Ms, Rat ICC/IF, WB 20 μl
ARG66285 anti-IL1 beta antibody [SQab1748] Mouse mAb Hu, Ms ELISA, FACS, ICC/IF, IHC-P, WB 20 μg
ARG62347 anti-beta Tubulin antibody [BT7R] Mouse mAb Chk, Hu, Mk, Ms, Rb, Rat Dot, ELISA, ICC/IF, WB 20 μg

Overview

Product Description Pyroptosis Antibody Panel is an all-in-one solution to make pyroptosis research easy and economic. This antibody panel comprises antibodies against pyroptosis key players Caspase-1, GSDMD, and IL1 beta. All three antibodies are good for detecting both pro- and cleaved proteins. Moreover, a loading control antibody against Tubulin is also included. This panel is the best solution for studying pyroptosis.

Related news:
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Target Name Pyroptosis
Alternate Names Pyroptosis antibody; GSDMD antibody; Caspase 1 antibody; beta Tubulin antibody; IL1 beta antibody

Properties

Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -333°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (16) Click the Picture to Zoom In

  • ARG62347 anti-beta Tubulin antibody [BT7R] WB image

    Western blot: 20 μg of PC3, MCF7, MDA-MD-231, K562, M. brain, R. brain, MCF7, HT29, 3T3 and Raw264.7 cell lysates stained with ARG62347 anti-beta Tubulin antibody [BT7R] at 1:3000 dilution.

  • ARG62347 anti-beta Tubulin antibody [BT7R] WB image

    Western blot: 20 µg of 293T, Mouse brain and Rat brain lysates stained with ARG62347 anti-beta Tubulin antibody [BT7R] at 1:10000 dilution.

  • ARG57293 anti-Caspase 1 antibody WB image

    Western blot: 10 µg of HeLa and 20 µg of Mouse brain lysates stained with ARG57293 anti-Caspase 1 antibody at 1:1000 dilution.

  • ARG66285 anti-IL1 beta antibody [SQab1748] WB image

    Western blot: 20 μg of A431 and HeLa cell lysates stained with ARG66285 anti-IL1 beta antibody [SQab1748] at 1:4000 dilution.

  • ARG57293 anti-Caspase 1 antibody WB image

    Western blot: 25 µg of cell lysate from PMA treated THP-1 cells were stained with ARG57293 anti-Caspase 1 antibody at 1:1000 dilution.

  • ARG57293 anti-Caspase 1 antibody ICC/IF image

    Immunofluorescence: Raw264.7 cells stained with ARG57293 anti-Caspase 1 antibody at 1:100 dilution.

  • ARG66285 anti-IL1 beta antibody [SQab1748] ICC/IF image

    Immunofluorescence: MCF7 cells stained with ARG66285 anti-IL1 beta antibody [SQab1748] at 1:200 dilution.

  • ARG41404 anti-GSDMD antibody ICC/IF image

    Immunofluorescence: RAW264.7 cells stained with ARG41404 anti-GSDMD antibody at 1:100 dilution.

  • ARG66285 anti-IL1 beta antibody [SQab1748] FACS image

    Flow Cytometry: HeLa cells stained with ARG66285 anti-IL1 beta antibody [SQab1748] at 1 µg/ml (red) and without antibody control (black).

  • ARG57293 anti-Caspase 1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse kidney tissue stained with ARG57293 anti-Caspase 1 antibody at 1:100 dilution.

  • ARG41404 anti-GSDMD antibody WB image

    Western blot: 25 µg of Mouse lung lysate stained with ARG41404 anti-GSDMD antibody at 1:1000 dilution.

  • ARG57293 anti-Caspase 1 antibody IP image

    Immunoprecipitation: 200 µg extracts of THP-1 cells Immunoprecipitated and stained with ARG57293 anti-Caspase 1 antibody at 1:1000 dilution.

  • ARG62347 anti-beta Tubulin antibody [BT7R] WB image

    Western blot: U87-MG and SW480 cell lysates stained with ARG62347 anti-beta Tubulin antibody [BT7R] at 1:1000 dilution.

  • ARG66285 anti-IL1 beta antibody [SQab1748] ICC/IF image

    Immunofluorescence: HeLa cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with ARG66285 anti-IL1 beta antibody [SQab1748] (green) at 1:200 dilution, and cell nuclei were stained with Hoechst 33342 (blue).

  • ARG66285 anti-IL1 beta antibody [SQab1748] WB image

    Western blot: 50 µg of Mouse liver lysate stained with ARG66285 anti-IL1 beta antibody [SQab1748] at 1:5000 dilution.

  • ARG66285 anti-IL1 beta antibody [SQab1748] WB image

    Western blot: 50 µg of Rat liver lysate stained with ARG66285 anti-IL1 beta antibody [SQab1748] at 1:5000 dilution.

Specific References

Curcumin Ameliorates White Matter Injury after Ischemic Stroke by Inhibiting Microglia/Macrophage Pyroptosis through NF- κ B Suppression and NLRP3 Inflammasome Inhibition

ARG41404: WB / Mouse

Yuanyuan Ran et al.
Oxid Med Cell Longev.,  (2021)

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Aronia Melanocarpa Polysaccharide Ameliotates Inflammation and Aging in Mice by Modulating AMPK/SIRT1/NF-κB Signaling Pathway and Gut Microbiota

ARG41404: WB; ARG66285: WB; ARG57293: WB / Mouse

Yingchun Zhao et al.
Preprint.,  (2021)

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Role of P2X7R in eosinophilic and non‑eosinophilic chronic rhinosinusitis with nasal polyps.

ARG66285: WB / Human

Yan Wang et al.
Mol Med Rep.,  (2021)

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Abscisic acid ameliorates oxidative stress, inflammation, and apoptosis in thioacetamide-induced hepatic fibrosis by regulating the NF-кB signaling pathway in mice.

ARG66285: WB / Mouse

Xueyan Chen et al.
Eur J Pharmacol.,  (2020)

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