ARG30002

Tag Internal Control Antibody Duo (His tag, GAPDH)

Cancer antibody; Controls and Markers antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG10112 anti-GAPDH antibody [6C5] Mouse mAb AGMK, Bb, Cat, Chk, Dog, Fsh, Hm, Hu, Mk, Ms, Pig, Rb, Rat, Xenopus laevis, Zfsh ELISA, ICC/IF, IHC-Fr, WB 50 μg
ARG62337 anti-6X His tag antibody [HIS.H8] Mouse mAb Other Dot, EMSA, ELISA, ICC/IF, IP, WB 50 μg

Overview

Product Description

His-tag, also known as hexa histidine-tag, 6xHis-tag or His6 tag , is a polyhistidine-tag (H-H-H-H-H-H) motif in proteins that consists of at least six histidine (His) residues at the N- or C-terminus of the protein. His-tag is used as a tag on many recombinant proteins for the purification or protein study in cells. GAPDH is often stably and constitutively expressed at high levels in most tissues and cells in cytoplasm. Therefore, GAPDH is a widely used loading control and cytoplasm marker for western blot (35-40kDa) and ICC/IF study. Usage note: S-nitrosylated GAPDH might translocate to nucleus especially for nitric oxide-related studies. In addition, the GAPDH expression level is up regulated under hypoxia condition. Therefore, GAPDH is not suitable for loading control and cytoplasm marker for oxygen-related and nitric oxide-related studies. To study protein function in cells, researchers could use anti-His tag antibody to recognize the His-tagged protein in the samples. Meanwhile, a good internal control is also important to control the loading of samples or the location of the intracellular organelles. ARG3002 Tag Internal control Duos (His tag, GAPDH), including an antibody reacts His-tag and an antibody recognizes GAPDH as a cytoplasm control and loading control is useful for His-tagged protein study for you.

Properties

Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Research Area Cancer antibody; Controls and Markers antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody

Images (9) Click the Picture to Zoom In

  • ARG10112 anti-GAPDH antibody [6C5] WB image

    Western blot: 1) U87-MG 2) HepG2 3) rat brain 4) rat heart 5) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:2000 dilution.

  • ARG10112 anti-GAPDH antibody [6C5] ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG10112 anti-GAPDH antibody [6C5] (green) at 1:200 dilution.

    Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)

  • ARG10112 anti-GAPDH antibody [6C5] WB image

    Western Blot: 1) HeLa, 2) NTERA-2, 3) A-431, 4) HepG2, 5) MCF-7, 6) NIH 3T3, 7) PC-12 and 8) COS-7 whole cell lysates stained with anti-GAPDH antibody [6C5] (ARG10112)

  • ARG62337 anti-6x His tag antibody [HIS.H8] WB image

    Western Blot: (1) untransfected control, (2) HEK293 cells transfected with His-tagged protein vector stained with ARG62337 anti-6x His tag antibody [HIS.H8]

  • ARG62337 anti-6x His tag antibody [HIS.H8] WB image

    Western Blot: a standard ladder containing five different His-tagged proteins stained with anti-His tag (HIS.H8 / EH158) mAb at 1:2000 (0.5 μg/mL) to compare between.

  • ARG10112 anti-GAPDH antibody [6C5] IP image

    Immunoprecipitation and western blot: 1) GAPDH (1 μg). 2) GAPDH IP from rat heart tissue extract. 3) Only GAPDH preincubated with Protein A Sepharose. 4) Only Protein A Sepharose stained with ARG10112 GAPDH antibody [6C5].


    Mixture of protein A-Sepharose with ARG10112 anti-GAPDH and tissue extract was incubated for 30 min at room temperature and precipitated by centrifugation. Pellet was washed with PBS, suspended in reducing electrophoresis sample buffer and heated for 5 minutes at 100ºC. After centrifugation supernatant was loaded on gel and proteins were separated by SDS electrophoresis.

  • ARG62337 anti-6x His tag antibody [HIS.H8] ICC/IF image

    TOP: untransfected control; BOTTOM: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue)

  • ARG10112 anti-GAPDH antibody [6C5] WB image

    Western blot: 1) U87-MG 2) MCF-7 3) A549 4) DU145 5) SW480 6) rat brain 7) rat stomach 8) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:5000 dilution.

  • ARG10112 anti-GAPDH antibody [6C5] WB image

    Western blot: 1) MCF-7 2) DU-145 3) A549 4) H1299 5) HCT116 6) HepG2 7) HUVEC stained with ARG10112 anti-GAPDH antibody [6C5] at 1:1000 dilution.