ARG30255

IKB alpha degradation Antibody Panel (IKK alpha, IKK beta, IKB alpha)

Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody
publication_link Publication1

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG54650 anti-IKK alpha antibody Rabbit pAb Hu ELISA, ICC/IF, WB 25 μg
ARG54686 anti-IKK beta antibody Rabbit pAb Hu ELISA, ICC/IF, WB 25 μg
ARG54710 anti-IKB alpha antibody Rabbit pAb Hu, Ms, Rat WB 50 μl
ARG65351 Goat anti-Rabbit IgG antibody (HRP) Goat pAb Rb ELISA, IHC-P, WB 50 μl

Overview

Product Description Rel or NF-kappaB (NF-kB) proteins comprise a family of structurally-related eukaryotic transcription factors that are involved in the control of a large number of normal cellular and organismal processes, such as immune and inflammatory responses, developmental processes, cellular growth, and apoptosis.
Once activated, IκB proteins are phosphorylated by IKKα and IKKβ, leading to the degradation of IκB proteins and nuclear translocation of NF-κB proteins. Hence, up-regulation of phospho-IKKα, phospho-IKKβ and down-regulation of IkB alpha is often associated with the activation of NF-kB downstream signaling pathway.
Hayden and Ghosh. (2012) Genes and Dev 26: 203-234
Baeuerle and Henkel. (1994) Ann Rev Immunol 12: 141-179
Target Name IKB alpha degradation
Alternate Names IKB alpha degradation antibody; IKK alpha antibody; IKK beta antibody; IKB alpha antibody

Properties

Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Full Name Antibody Panel for IKB alpha degradation (IKK alpha, IKK beta, IKB alpha)
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody

Images (7) Click the Picture to Zoom In

  • ARG54650 anti-IKK alpha antibody WB image

    Western blot: 30 μg of HeLa cell lysates 1) untreated or 2) treated with 5 ng/ml TNF-a for 5 min, stained with ARG54650 anti-IKK alpha antibody at 1:500 dilution.

  • ARG54686 anti-IKK beta antibody WB image

    Western blot: 30 μg of HeLa cell lysates 1) untreated or 2) treated with 5 ng/ml TNF-a for 5 min, stained with ARG54686 anti-IKK beta antibody at 1:500 dilution.

  • ARG54710 anti-IKB alpha antibody WB image

    Western blot: 30 µg of HeLa cells treated with 5, 10 or 20 ng/ml of TNF alpha at 10, 20 or 30 mins. The blots were stained with ARG54710  anti-IKB alpha antibody at 1:500 dilution. The ARG10112 anti-GAPDH antibody [6C5] was used as loading control.

  • ARG54650 anti-IKK alpha antibody ICC/IF image

    Immunocytochemistry: Jurkat cells stained with ARG54650 anti-IKK alpha antibody at 1μg/ml.

  • ARG54650 anti-IKK alpha antibody ICC/IF image

    Immunofluorescence: Jurkat cells stained with ARG54650 anti-IKK alpha antibody at 10 μg/ml.

  • ARG54686 anti-IKK beta antibody ICC/IF image

    Immunocytochemistry: HeLa cells stained with ARG54686 anti-IKK beta antibody at 10 μg/ml.

  • ARG54686 anti-IKK beta antibody ICC/IF image

    Immunofluorescence: Hela cells stained with ARG54686 anti-IKK beta antibody at 10 μg/ml.

Specific References

Caffeic acid phenethyl ester down-regulates claudin-2 expression at the transcriptional and post-translational levels and enhances chemosensitivity to doxorubicin in lung adenocarcinoma A549 cells.

ARG54710: WB / Human

Sonoki Hiroyuki et al.
The Journal of Nutritional Biochemistry.,  (2018)

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