ARG30259

Loading Controls for Cytoplasmic / Nuclear Fractions Antibody Panel

Cancer antibody; Controls and Markers antibody; Gene Regulation antibody; Immune System antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
publication_link Publication55

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG54003 anti-COX4 antibody Mouse mAb Goat, Hm, Hu, Mk, Ms, Rat FACS, ICC/IF, IHC-P, IP, WB 25 μl
ARG65681 anti-Histone H3 antibody Mouse mAb Hu, Ms, Rat ICC/IF, IHC-P, IP, WB 25 μg
ARG10112 anti-GAPDH antibody [6C5] Mouse mAb AGMK, Bb, Cat, Chk, Dog, Fsh, Hm, Hu, Mk, Ms, Pig, Rb, Rat, Xenopus laevis, Zfsh ELISA, ICC/IF, IHC-Fr, WB 25 μg
ARG65350 Goat anti-Mouse IgG antibody (HRP) Goat pAb Ms ELISA, IHC-P, WB 50 μl

Overview

Target Name Loading Controls for Cytoplasmic / Nuclear Fractions
Alternate Names Loading Controls for Cytoplasmic / Nuclear Fractions antibody; GAPDH antibody; COX4 antibody; Histone H3 antibody

Properties

Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Full Name Antibody Panel for Loading Controls for Cytoplasmic / Nuclear Fractions
Research Area Cancer antibody; Controls and Markers antibody; Gene Regulation antibody; Immune System antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody

Images (18) Click the Picture to Zoom In

  • ARG10112 anti-GAPDH antibody [6C5] WB image

    Western blot: 1) U87-MG 2) HepG2 3) rat brain 4) rat heart 5) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:2000 dilution.

  • ARG65681 anti-Histone H3 antibody IP image

    Immunoprecipitation: 1) HeLa cell lysate stained with ARG65681 anti-Histone H3 antibody and 2) IP product immunoprecipitated by ARG65681 anti-Histone H3 antibody at 1:200 dilution.

  • ARG10112 anti-GAPDH antibody [6C5] ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG10112 anti-GAPDH antibody [6C5] (green) at 1:200 dilution.

    Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)

  • ARG54003 anti-COX4 antibody ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG54003 anti-COX4 antibody (green) at 1:150 dilution.

    Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)

  • ARG65681 anti-Histone H3 antibody ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG65681 anti-Histone H3 antibody at 1:100 dilution. Left: primary antibody (green). Right: Merge (primary antibody and DAPI).

    Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)

  • ARG65681 anti-Histone H3 antibody WB image

    Western blot: 20 µg of HeLa, Mouse brain and Rat brain lysates stained with ARG65681 anti-Histone H3 antibody at 1:2000 dilution.

  • ARG10112 anti-GAPDH antibody [6C5] WB image

    Western blot: 1) U87-MG 2) MCF-7 3) A549 4) DU145 5) SW480 6) rat brain 7) rat stomach 8) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:5000 dilution.

  • ARG10112 anti-GAPDH antibody [6C5] WB image

    Western blot: 1) MCF-7 2) DU-145 3) A549 4) H1299 5) HCT116 6) HepG2 7) HUVEC stained with ARG10112 anti-GAPDH antibody [6C5] at 1:1000 dilution.

  • ARG10112 anti-GAPDH antibody [6C5] WB image

    Western Blot: 1) HeLa, 2) NTERA-2, 3) A-431, 4) HepG2, 5) MCF-7, 6) NIH 3T3, 7) PC-12 and 8) COS-7 whole cell lysates stained with anti-GAPDH antibody [6C5] (ARG10112)

  • ARG65681 anti-Histone H3 antibody WB image

    Western blot: 1) HeLa, 2) Raw, 3) Mouse brain tissue, and 4) Rat brain tissue lysates stained with ARG65681 anti-Histone H3 antibody at 1:5000 dilution.

  • ARG54003 anti-COX4 antibody FACS image

    Flow Cytometry: K562 cells stained with ARG54003 anti-COX4 antibody at 1:100 dilution (right histogram) or isotype control (left histogram), followed by incubation with FITC labelled secondary antibody.

  • ARG54003 anti-COX4 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human colorectal carcinoma stained with ARG54003 anti-COX4 antibody at 1:50 dilution. Antigen Retrieval: High-pressure and temperature Citrate buffer (pH 6.0).

  • ARG65681 anti-Histone H3 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat testis tissue stained with ARG65681 anti-Histone H3 antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min. Secondary antibody was diluted at 1:200 (RT, 30 min).

    Negative control was used by secondary antibody only.

  • ARG10112 anti-GAPDH antibody [6C5] IP image

    Immunoprecipitation and western blot: 1) GAPDH (1 μg). 2) GAPDH IP from rat heart tissue extract. 3) Only GAPDH preincubated with Protein A Sepharose. 4) Only Protein A Sepharose stained with ARG10112 GAPDH antibody [6C5].


    Mixture of protein A-Sepharose with ARG10112 anti-GAPDH and tissue extract was incubated for 30 min at room temperature and precipitated by centrifugation. Pellet was washed with PBS, suspended in reducing electrophoresis sample buffer and heated for 5 minutes at 100ºC. After centrifugation supernatant was loaded on gel and proteins were separated by SDS electrophoresis.

  • ARG65681 anti-Histone H3 antibody IP image

    Immunoprecipitation: 1) HeLa cell lysate stained with ARG65681 anti-Histone H3 antibody and 2) IP product immunoprecipitated by ARG65681 anti-Histone H3 antibody at 1:200 dilution.

  • ARG54003 anti-COX4 antibody IP image

    Immunoprecipitation: HeLa cell lysates were immunoprecipitated and stained with ARG54003 anti-COX4 antibody.

  • ARG54003 anti-COX4 antibody WB image

    Western blot: 20 µg of HeLa, Mouse brain and Rat brain lysates stained with ARG54003 anti-COX4 antibody at 1:1000 dilution.

  • ARG54003 anti-COX4 antibody ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG54003 anti-COX4 antibody (green) at 1:150 dilution.

    Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)

Specific References

Downregulation of lncRNA NEAT1 alleviates sepsis-induced acute kidney injury

ARG10112: WB / Human

Yuhua Zhou et al.
Central European Journal of Immunology,  (2022)

publication_link

 

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Curcumin attenuates renal ischemia reperfusion injury via JNK pathway with the involvement of p300/CBP-mediated histone acetylation

ARG10112: WB / Rat

Lu Yang et al.
Korean J Physiol Pharmacol.,  (2021)

publication_link

 

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7,8-dihydroxyflavone ameliorates motor deficits via regulating autophagy in MPTP-induced mouse model of Parkinson's disease

ARG10112: WB / Mouse

Li Zuo et al.
Cell Death Discov.,  (2021)

publication_link

 

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Evaluation of Class IIa Histone Deacetylases Expression and In Vivo Epigenetic Imaging in a Transgenic Mouse Model of Alzheimer's Disease.

ARG10112: WB / Human

Yi-An Chen et al.
International Journal of Molecular Sciences.,  (2021)

publication_link

 

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Cardiac-Specific Gene TNNI3 as a Potential Oncogene for Kidney Cancer and Its Involvement in Wnt Signaling Pathway.

ARG10112: WB / Human

Weian Zhao et al.
Preprint.,  (2021)

publication_link

 

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Inactivation of the tumor suppressor p53 by long noncoding RNA RMRP.

ARG65350: WB / Mouse

Yajie Chen et al.
Proc Natl Acad Sci.,  (2021)

publication_link

 

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Blocking the CCL5-CCR5 Axis Using Maraviroc Promotes M1 Polarization of Macrophages Cocultured with Irradiated Hepatoma Cells.

ARG65350: WB / Mouse

Yuan Zhuang et al.
J Hepatocell Carcinoma.,  (2021)

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Repurposing the FDA-approved anticancer agent ponatinib as a fluconazole potentiator by suppression of multidrug efflux and Pma1 expression in a broad spectrum of yeast species.

ARG65350: WB / Mouse

Lin Liu et al.
Microb Biotechnol.,  (2021)

publication_link

 

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Mesenchymal Stem Cell-Derived Exosomes Ameliorate Alzheimer's Disease Pathology and Improve Cognitive Deficits.

ARG10112: WB / Human

Yi-An Chen et al.
Biomedicines.,  (2021)

publication_link

 

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Decidual NR2F2-Expressing CD4 + T Cells Promote TH2 Transcriptional Program During Early Pregnancy.

ARG65350: WB / Mouse

Yikong Lin et al.
Front Immunol.,  (2021)

publication_link

 

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MicroRNA-145 Delay Plaque Erosion by Down-Regulating TGFβr Ii and Suppressing Autophagy of Vascular Smooth Muscle Cells.

ARG65350: WB / Mouse

Yunjuan Jiao et al.
Preprint.,  (2021)

publication_link

 

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Circ_0081572 inhibits the progression of periodontitis through regulating the miR-378h/RORA axis.

ARG10112: WB / Human

Jing Wang et al.
Arch Oral Biol.,  (2021)

publication_link

 

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Design, Synthesis, and Activity Evaluation of Novel Acyclic Nucleosides as Potential Anticancer Agents In Vitro and In Vivo.

ARG65350: WB / Mouse

Er-Jun Hao et al.
J Med Chem.,  (2021)

publication_link

 

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Intermittent pressure imitating rolling manipulation ameliorates injury in skeletal muscle cells through oxidative stress and lipid metabolism signalling pathways.

ARG65350: WB / Mouse

Li-Juan Zhao et al.
Gene.,  (2021)

publication_link

 

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Structural Basis of VSIG3: The Ligand for VISTA.

ARG10112: WB / Human

Xiaoxue Xie et al.
Front Immunol.,  (2021)

publication_link

 

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The Phosphorylation Status of Drp1-Ser637 by PKA in Mitochondrial Fission Modulates Mitophagy via PINK1/Parkin to Exert Multipolar Spindles Assembly during Mitosis.

ARG10112: WB / Human

Huey-Jiun Ko et al.
Biomolecules.,  (2021)

publication_link

 

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M351-0056 is a novel low MW compound modulating the actions of the immune-checkpoint protein VISTA.

ARG10112: WB / Human, Mouse

Xinlei Hu et al.
Br J Pharmacol.,  (2021)

publication_link

 

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SCM-198 protects endometrial stromal cells from oxidative damage through Bax/Bcl-2 and ERK signaling pathways.

ARG10112: WB / Human

Yunyun Li et al.
Acta Biochim Biophys Sin.,  (2019)

publication_link

 

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Excessive fibroblast growth factor 23 promotes renal fibrosis in mice with type 2 cardiorenal syndrome.

ARG10112: WB / Mouse, Rat

Huixin Hao et al.
Aging (Albany NY).,  (2021)

publication_link

 

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BCL7C suppresses ovarian cancer growth by inactivating mutant p53.

ARG65350: WB / Mouse

Canhua Huang et al.
J Mol Cell Biol.,  (2020)

publication_link

 

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ERK activation precedes Purkinje cell loss in mice with Spinocerebellar ataxia type 17.

ARG10112: WB / Mouse

Chia-Wei Lin et al.
Neurosci Lett.,  (2020)

publication_link

 

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Ubiquitin ligase DTX3 empowers mutant p53 to promote ovarian cancer development.

ARG65350: WB / Mouse

Shanshan Wang et al.
Genes & Diseases.,  (2020)

publication_link

 

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Synthesis and anti-inflammatory activity of saponin derivatives of δ-oleanolic acid.

ARG10112: WB / Human

Liu Liu et al.
European Journal of Medicinal Chemistry.,  (2020)

publication_link

 

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miR-765 Impairs Pancreatic β-cell Function by Targeting PDX1 in type 2 Diabetes.

ARG65350: WB / Mouse

Li Zheng et al.
Preprint.,  (2020)

publication_link

 

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CLC-3 and SOX2 regulate the cell cycle in DU145 cells.

ARG65350: WB / Mouse

Jiahong Chen et al.
Oncology Letters.,  (2020)

publication_link

 

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SMARCB1 Promotes Ubiquitination and Degradation of NR4A3 via Direct Interaction Driven by ROS in Vascular Endothelial Cell Injury.

ARG10112, ARG65350: WB / Human, Mouse, Rat

Bingzheng Lu et al.
Oxidative Medicine and Cellular Longevity.,  (2020)

publication_link

 

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Study of isolation and characterization of extracellular vesicles in senescent tumor cells induced by chemotherapeutics.

ARG10112: WB / Human

Qi Zhou et al.
Chinese Journal of Immunology.,  (2020)

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Increased expression levels of inflammatory cytokines and adhesion molecules in lipopolysaccharide-induced acute inflammatory apoM-/- mice.

ARG10112: WB / Mouse

Yuanping Shi et al.
Molecular Medicine Reports.,  (2020)

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Discovery of AdipoRon analogues as novel AMPK activators without inhibiting mitochondrial complex I.

ARG10112: WB / Human, Mouse

Geng Sun et al.
Eur J Med Chem.,  (2020)

publication_link

 

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High expression of FUSE binding protein 1 in breast cancer stimulates cell proliferation and diminishes drug sensitivity.

ARG65350: WB / Mouse

Liu Wei et al.
Int J Oncol.,  (2020)

publication_link

 

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Discovery of AdipoRon analogues as novel AMPK activators without inhibiting mitochondrial complex I.

ARG10112: WB / Human, Mouse

Sun Geng et al.
European Journal of Medicinal Chemistry.,  (2020)

publication_link

 

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Prokaryotic expression and immunogenicity analysis of Clostridium difficile binary toxin A.

ARG65350: WB / Mouse

Huang Yingfeng et al.
检验医学 (Laboratory Medicine),  (2020)

publication_link

 

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A single-component light sensor system allows highly tunable and direct activation of gene expression in bacterial cells.

ARG65350: WB / Mouse

Li Xie et al.
Nucleic Acids Res.,  (2020)

publication_link

 

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Cardiac troponin I R193H mutant interacts with HDAC1 to repress phosphodiesterase 4D expression in cardiomyocytes.

ARG10112: WB / Mouse

Zhao Weian et al.
Genes & Diseases.,  (2020)

publication_link

 

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Codelivery of Anti-PD-1 Antibody and Paclitaxel with Matrix Metalloproteinase and pH Dual-Sensitive Micelles for Enhanced Tumor Chemoimmunotherapy.

ARG65350: WB, IHC-P / Mouse

Su Zhenwei et al.
Small.,  (2020)

publication_link

 

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Ubiquitin ligase TRIM71 suppresses ovarian tumorigenesis by degrading mutant p53.

ARG65350: WB, IHC-P / Mouse

Chen Yajie et al.
Cell Death and Disease.,  (2019)

publication_link

 

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Down-regulation of HTR1A-modulated ACC activation contributes to stress-induced visceral hyperalgesia in rats.

ARG65350: WB / Mouse

Yi Lisha et al.
Neurogastroenterol Motil.,  (2019)

publication_link

 

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Effects of perfluorooctane on the retina as a short-term and small amounts remnant in rabbits.

ARG65350: IHC-P / Mouse

Li Qing-Chen et al.
Int J Ophthalmol.,  (2019)

publication_link

 

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Epigenetic regulation of phosphodiesterase 4d in restrictive cardiomyopathy mice with cTnI mutations.

ARG10112: WB / Mouse

Zhao Weian et al.
Sci China Life Sci.,  (2019)

publication_link

 

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Icariin suppresses cell cycle transition and cell migration in ovarian cancer cells.

ARG65350: WB / Mouse

Wang Pengzhen et al.
Oncol Rep.,  (2019)

publication_link

 

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Tumor-associated neutrophils induce EMT by IL-17a to promote migration and invasion in gastric cancer cells.

ARG10112: WB / Human

Li Sen et al.
J Exp Clin Cancer Res.,  (2019)

publication_link

 

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Genetic labeling reveals temporal and spatial expression pattern of D2 dopamine receptor in rat forebrain.

ARG10112: WB / Rat

Yu Qing et al.
Brain Struct Funct.,  (2019)

publication_link

 

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AdipoRon prevents l-thyroxine or isoproterenol-induced cardiac hypertrophy through regulating the AMPK-related pathway.

ARG10112: WB / Rat

Hu Xinlei et al.
Acta Biochim Biophys Sin (Shanghai).,  (2018)

publication_link

 

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The effect of melatonin on cardio fibrosis in juvenile rats with pressure overload and deregulation of HDACs.

ARG10112: WB / Rat

Wu Yao et al.
Korean J Physiol Pharmacol.,  (2018)

publication_link

 

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Epigallocatechin-3 gallate prevents pressure overload-induced heart failure by up-regulating SERCA2a via histone acetylation modification in mice.

ARG10112: WB / Mouse

Liu Lifei et al.
PLoS One.,  (2018)

publication_link

 

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MIR4532 gene variant rs60432575 influences the expression of KCNJ11 and the sulfonylureas-stimulated insulin secretion.

ARG65350: WB / Mouse

Chen Zhang-Ren et al.
Endocrine.,  (2018)

publication_link

 

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Knockdown of LGALS12 inhibits porcine adipocyte adipogenesis via PKA-Erk1/2 signaling pathway

ARG65350: WB / Mouse

Wu Wenjing et al.
Acta Biochim Biophys Sin.,  (2018)

publication_link

 

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抗菌肽Sublancin增强小鼠获得性免疫的研究

ARG65350: ELISA / Mouse

张晓雅 et al.
动物营养学报,  (2018)

publication_link

 

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Treatment of diabetic mice with A Combination of Ketogenic Diet and Aerobic Exercise via modulations of PPARs gene programs.

ARG65350: WB / Mouse

Zhang Qiang et al.
PPAR Research.,  (2018)

publication_link

 

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Checking transfer efficiency and equal loading via qualitative optical way in western blotting.

ARG10112: WB / Human

Gong JH et al.
Electrophoresis,  (2017)

publication_link

 

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Cardiac Protection of Valsartan on Juvenile Rats with Heart Failure by Inhibiting Activity of CaMKII via Attenuating Phosphorylation

ARG10112: WB / Rat

Yao Wu et al.
BioMed Research International.,  (2017)

publication_link

 

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Hydrogen sulfide protects against endoplasmic reticulum stress and mitochondrial injury in nucleus pulposus cells and ameliorates intervertebral disc degeneration.

ARG10112: WB / Human

Xu D et al.
Pharmacol Res.,  (2017)

publication_link

 

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Study on the inhibition of hyperthermic CO₂ pneumoperitoneum on the proliferation and migration of colon cancer cells and its mechanism.

ARG65350: WB / Mouse

Zhao J et al.
Oncol Rep.,  (2016)

publication_link

 

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Acute hyperglycemia suppresses left ventricular diastolic function and inhibits autophagic flux in mice under prohypertrophic stimulation.

ARG10112: WB / Mouse

Xie J et al.
Cardiovasc Diabetol.,  (2016)

publication_link

 

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Reciprocal regulation between O-GlcNAcylation and tribbles pseudokinase 2 (TRIB2) maintains transformative phenotypes in liver cancer cells.

ARG10112: WB / Human

Yao B et al.
Cell Signal.,  (2016)

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