ARG54150

anti-STAT3 antibody

anti-STAT3 antibody for Western blot and Hamster,Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
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anti-STAT3 antibody

Mouse Monoclonal antibody recognizes STAT3

ARG54150 Arigo biolaboratories
4.3 out of 5 based on 3
anti-STAT3 antibody is rated 4.3 out of 5 by 3.

anti-STAT3 antibody

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Overview

Product Description

Mouse Monoclonal antibody recognizes STAT3

Tested Reactivity Hu, Ms, Rat, Hm
Tested Application WB
Host Mouse
Clonality Monoclonal
Isotype IgG1
Target Name STAT3
Antigen Species Human
Immunogen Purified recombinant human STAT3 protein fragments expressed in E.coli
Conjugation Un-conjugated
Full Name signal transducer and activator of transcription 3 (acute-phase response factor)
Alternate Names ADMIO; APRF; HIES; Acute-phase response factor; Signal transducer and activator of transcription 3

Application Instructions

Application Suggestion
Tested Application Dilution
WB 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer Ascites
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 20848 Mouse STAT3

GeneID: 25125 Rat STAT3

GeneID: 6774 Human STAT3

Gene Symbol STAT3
Gene Full Name signal transducer and activator of transcription 3 (acute-phase response factor)
Background Signal transducer and transcription activator that mediates cellular responses to interleukins,KITLG/SCF and other growth factors.May mediate cellular responses to activated FGFR1,FGFR2,FGFR3 and FGFR4.Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes.Activated by IL31 through IL31RA.Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity.Plays an important role in host defense in methicillin-resistant S.aureus lung infection by regulating the expression of the antimicrobial lectin REG3G by similarity.
Function Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity. Plays an important role in host defense in methicillin-resistant S.aureus lung infection by regulating the expression of the antimicrobial lectin REG3G (By similarity). [UniProt]
Cellular Localization Cytoplasm. Nucleus. Note: Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.
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STAT3 antibodies; Anti-Mouse IgG secondary antibodies;
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Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
PTM Tyrosine phosphorylated upon stimulation with EGF. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Activated through tyrosine phosphorylation by BMX. Tyrosine phosphorylated in response to IL6, IL11, LIF, CNTF, KITLG/SCF, CSF1, EGF, PDGF, IFN-alpha, LEP and OSM. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus. Upon LPS challenge, phosphorylated within the nucleus by IRAK1. Upon erythropoietin treatment, phosphorylated on Ser-727 by RPS6KA5. Phosphorylation at Tyr-705 by PTK6 or FER leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates IL6/interleukin-6 signaling.
Acetylated on lysine residues by CREBBP. Deacetylation by LOXL3 leads to disrupt STAT3 dimerization and inhibit STAT3 transcription activity (PubMed:28065600). Oxidation of lysine residues to allysine on STAT3 preferentially takes place on lysine residues that are acetylated (PubMed:28065600).
Some lysine residues are oxidized to allysine by LOXL3, leading to disrupt STAT3 dimerization and inhibit STAT3 transcription activity (PubMed:28065600). Oxidation of lysine residues to allysine on STAT3 preferentially takes place on lysine residues that are acetylated (PubMed:28065600).

Images (1) Click the Picture to Zoom In

  • ARG54150 anti-STAT3 antibody WB image

    Western blot: 20 µg of HeLa cell lysate stained with ARG54150 anti-STAT3 antibody at 1:1000 dilution.

Customer's Feedback

nuts_pic      Excellent

Review for anti-STAT3 antibody

Application:WB

Sample:HeLa

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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nuts_pic      Excellent

Review for anti-STAT3 antibody

Application:WB

Sample:HeLa

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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nuts_pic      Average

Review for anti-STAT3 antibody

Application:WB

Sample:MCF-7

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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Specific References

Mesenchymal stem cells enhance autophagy of human intrahepatic biliary epithelial cells in vitro.

WB / Human

Zhu Yun et al.
Cell Biochem Funct.,  (2018)

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IL-6 receptor blockade corrects defects of XIAP-deficient regulatory T cells.

WB / Mouse

Hsieh Wan-Chen et al.
Nat Commun.,  (2018)

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Cancer-associated fibroblasts treated with cisplatin facilitates chemoresistance of lung adenocarcinoma through IL-11/IL-11R/STAT3 signaling pathway.

WB / Human

Tao L et al.
Sci Rep.,  (2016)

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