anti-TNF alpha antibody [2C8]

anti-TNF alpha antibody [2C8] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Neutralizing,Western blot and Human,Mouse

Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody
publication_link Publication5


Product Description

Mouse Monoclonal antibody [2C8] recognizes TNF alpha

Tested Reactivity Hu, Ms
Tested Application ELISA, FACS, ICC/IF, IHC-Fr, IHC-P, Neut, WB
Host Mouse
Clonality Monoclonal
Clone 2C8
Isotype IgG1
Target Name TNF alpha
Antigen Species Human
Immunogen Human recombinant tumor necrosis factor of alpha type
Conjugation Un-conjugated
Alternate Names Tumor necrosis factor ligand superfamily member 2; DIF; Cachectin; ICD2; ICD1; N-terminal fragment; TNF-a; TNFA; TNFSF2; TNF-alpha; Tumor necrosis factor; NTF

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent. (capture - detection): 2C8 - F6C5.
FACS1µg for 10^6 cells
ICC/IF10-20 µg/ml
IHC-Fr10-20 µg/ml
IHC-P10-20 µg/ml
Application Note Neutralizing: The clone [2C8] inhibit biological activity of TNF-alpha. The antibody concentration is depending on the test design from 10 ng/ml to 5 µg/ml.
ELISA: The clone 2C8 can be used as a capture antibody in sandwich ELISA in combination with a tracer/detection antibody clone [F6C5] (Cat. No.: ARG10441).
IHC-P: Antigen Retrieval: Boil tissue section in EDTA pH8.0 or pH9.0.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Protein A affinity purified.
Buffer PBS (pH 7.4) and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 21926 Mouse TNF

GeneID: 7124 Human TNF

Swiss-port # P01375 Human Tumor necrosis factor

Swiss-port # P06804 Mouse Tumor necrosis factor

Gene Symbol TNF
Gene Full Name tumor necrosis factor
Background This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
Function Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208).
The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. [UniProt]
Highlight Related products:
TNF alpha antibodies; TNF alpha ELISA Kits; TNF alpha Duos / Panels; TNF alpha recombinant proteins; Anti-Mouse IgG secondary antibodies;
Related news:
HMGB1 in inflammation
Inflammatory Cytokines
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 26 kDa
PTM The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.

Images (1) Click the Picture to Zoom In

  • ARG10158 anti-TNF alpha antibody [2C8] IHC-Fr image

    Immunohistochemistry: Frozen section of human joint from a patient with rheumatoid arthritis stained with ARG10158 anti-TNF alpha antibody [2C8].

Specific References

Paeoniflorin ameliorates diabetic liver injury by targeting the TXNIP-mediated NLRP3 inflammasome in db/db mice

IHC-P, WB / Mouse

Anli Wang et al.
Int Immunopharmacol.,  (2022)




Facile open-well immunofluorescence enhancement with coplanar-electrodes-enabled optoelectrokinetics and magnetic particles

ELISA (Capture) / Human

Wei-Long Chen et al.
Biosens Bioelectron.,  (2021)




Bruton's tyrosine kinase regulates macrophage-induced inflammation in the diabetic kidney via NLRP3 inflammasome activation.

WB / Mouse

Jing Zhao et al.
Int J Mol Med.,  (2021)




Enhanced Bruton's tyrosine kinase activity in the kidney of patients with IgA nephropathy.

IHC-P / Human

Jie Wei et al.
Int Urol Nephrol.,  (2021)




TAB1 regulates glycolysis and activation of macrophages in diabetic nephropathy.

WB, IHC-P / Mouse

Hanxu Zeng et al.
Inflamm Res.,  (2020)